Isolating Chromosomes of the Komodo Dragon: New Tools for Comparative Mapping and Sequence Assembly | Zendy

Alessio Iannucci | Zendy; Marie Altmanová | Zendy; Claúdio Ciofi | Zendy; M.A. FergusonSmith | Zendy; Jorge C. Pereira | Zendy; Ivan Rehák | Zendy; Roscoe Stanyon | Zendy; Petr Velenský | Zendy; Michail Rovatsos | Zendy; Lukáš Kratochvíl | Zendy; Martina Pokorná | Zendy

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Isolating Chromosomes of the Komodo Dragon: New Tools for Comparative Mapping and Sequence Assembly

Author(s) -

Alessio Iannucci,

Marie Altmanová,

Claúdio Ciofi,

M.A. FergusonSmith,

Jorge C. Pereira,

Ivan Rehák,

Roscoe Stanyon,

Petr Velenský,

Michail Rovatsos,

Lukáš Kratochvíl,

Martina Pokorná

Publication year - 2019

Publication title -

cytogenetic and genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.571

H-Index - 88

ISSN - 1424-8581

DOI - 10.1159/000496171

Subject(s) - biology , karyotype , chromosome , genetics , dna sequencing , chromosome 21 , chromosome 22 , dna , gene

We developed new tools to build a high-quality chromosomal map of the Komodo dragon (Varanus komodoensis) available for cross-species phylogenomic analyses. First, we isolated chromosomes by flow sorting and determined the chromosome content of each flow karyotype peak by FISH. We then isolated additional Komodo dragon chromosomes by microdissection and amplified chromosome-specific DNA pools. The chromosome-specific DNA pools can be sequenced, assembled, and mapped by next-generation sequencing technology. The chromosome-specific paint probes can be used to investigate karyotype evolution through cross-species chromosome painting. Overall, the set of chromosome-specific DNA pools of V. komodoensis provides new tools for detailed phylogenomic analyses of Varanidae and squamates in general.

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