Open AccessEvaluation of Diagnostic Tests by Evanescence Biosensor Technology for Rapid Phenotyping of the Human Platelet Alloantigens 1a and 5b
Author(s) -
Yves Mérieux,
Celestine Schwab,
Maurine Saint-Cyr,
Gabi Rink,
Claudio Rhyner,
Manfred Schawaller,
Peter Bugert
Publication year - 2018
Publication title -
transfusion medicine and hemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 39
eISSN - 1660-3818
pISSN - 1660-3796
DOI - 10.1159/000493556
Subject(s) - genotyping , genotype , taqman , single nucleotide polymorphism , antigen , neonatal alloimmune thrombocytopenia , platelet , microbiology and biotechnology , immunology , platelet membrane glycoprotein , biology , polymerase chain reaction , gene , genetics , pregnancy , fetus
The human platelet alloantigens (HPA) HPA-1a and HPA-5b are located on glycoproteins on the platelet surface and are the most relevant to cause neonatal alloimmune thrombocytopenia (NAIT). The antigens are defined by single nucleotide polymorphisms (SNPs) in the glycoprotein genes, and the antigen status can be determined by genotyping the SNPs. However, genotyping is time-consuming and costly depending on the method and sample throughput. Here, we tested the reliability of the evanescence wave based fluorescence (EVA) biosensor technology for the rapid phenotyping of the HPA-1a and HPA-5b antigens on blood donor samples in two laboratories.