Open AccessMir-382 Promotes Differentiation of Rat Liver Progenitor Cell WB-F344 by Targeting Ezh2
Author(s) -
Yan Zheng,
Jiansheng Zhou,
Xuebo Li,
Guangtao Xu,
Mingliang Jin,
Ruilin Shen,
Ruibing Su,
Shuyu Zhan,
Baoyue Ding,
Mingguang Jia,
Yuzhong Cui,
Xiaojun Yu
Publication year - 2018
Publication title -
cellular physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.486
H-Index - 87
eISSN - 1421-9778
pISSN - 1015-8987
DOI - 10.1159/000492654
Subject(s) - progenitor cell , hepatocyte , cellular differentiation , cell , biology , mir 122 , microrna , microbiology and biotechnology , cancer research , ezh2 , transfection , chemistry , stem cell , cell culture , gene expression , biochemistry , in vitro , gene , genetics
Background/Aims: Liver progenitor cells (LPCs) were considered as a promising hepatocyte source of cell therapy for liver disease due to their self-renewal and differentiation capacities, while little is known about the mechanism of LPC differentiate into hepatocytes. This study aims to explore the effect of miR-382, a member of Dlk1-Dio3 microRNA cluster, during hepatic differentiation from LPCs. Methods: In this study, we used rat liver progenitor cell WB-F344 as LPC cell model and HGF as inducer to simulate the process of LPCs hepatic differentiation, then microRNAs were quantified by qPCR. Next, WB-F344 cell was transfected with miR-382 mimics, then hepatocyte cell trait was characterized by multiple experiments, including that periodic acid schiff staining and cellular uptake and excretion of indocyanine green to evaluate the hepatocellular function, qPCR and Western Blotting analysis to detect the hepatocyte-specific markers (ALB, Ttr, Apo E and AFP) and transmission electron microscopy to observe the hepatocellular morphology. Moreover, Luciferase reporter assay was used to determine whether Ezh2 is the direct target of miR-382. Results: We found that miR-382 increased gradually and was inversely correlated with the potential target, Ezh2, during WB-F344 hepatic differentiation. In addition, functional studies indicated that miR-382 increased the level of hepatocyte-specific genes. Conclusions: This study demonstrates that miR-382 may be a novel regulator of LPCs differentiation by targeting Ezh2.