Open AccessComparison of SureSelect and Nextera Exome Capture Performance in Single-Cell Sequencing
Author(s) -
Wendy J. Huss,
Qiang Hu,
Sean T. Glenn,
Kalyan J. Gangavarapu,
Jianmin Wang,
Jesse Luce,
Paul K. Quinn,
Elizabeth Brese,
Fenglin Zhan,
Jeffrey M. Conroy,
György Paragh,
Barbara A. Foster,
Carl Morrison,
Song Liu,
Lei Wei
Publication year - 2018
Publication title -
human heredity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.423
H-Index - 62
eISSN - 1423-0062
pISSN - 0001-5652
DOI - 10.1159/000490506
Subject(s) - exome sequencing , exome , biology , genetics , computational biology , dna sequencing , mutation , dna , gene
Advances in single-cell sequencing provide unprecedented opportunities for clinical examination of circulating tumor cells, cancer stem cells, and other rare cells responsible for disease progression and drug resistance. On the genomic level, single-cell whole exome sequencing (scWES) started to gain popularity with its unique potentials in characterizing mutational landscapes at a single-cell level. Currently, there is little known about the performance of different exome capture kits in scWES. Nextera rapid capture (NXT; Illumina, Inc.) has been the only exome capture kit recommended for scWES by Fluidigm C1, a widely accessed system in single-cell preparation.