Open AccessOocyte Cytoplasmic Gas6 and Heparan Sulfate (HS) are Required to Establish the Open Chromatin State in Nuclei During Remodeling and Reprogramming
Author(s) -
KyeoungHwa Kim,
Eun Young Kim,
Suyeon Lee,
Jina Ko,
KyungAh Lee
Publication year - 2017
Publication title -
cellular physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.486
H-Index - 87
eISSN - 1421-9778
pISSN - 1015-8987
DOI - 10.1159/000486221
Subject(s) - reprogramming , microbiology and biotechnology , cytoplasm , oocyte , heparan sulfate , chromatin , chemistry , chromatin remodeling , biology , biochemistry , embryo , cell , dna
Previously, we found that silencing of growth arrest-specific gene 6 (Gas6) in oocytes impaired cytoplasmic maturation, resulting in failure of sperm chromatin decondensation (SCD) and pronuclear (PN) formation after fertilization. Thus, we conducted this study to determine the effect of Gas6 RNAi on downstream genes and to elucidate the working mechanism of Gas6 on oocyte cytoplasmic maturation and SCD.