Open AccessPermanently Hypoxic Cell Culture Yields Rat Bone Marrow Mesenchymal Cells with Higher Therapeutic Potential in the Treatment of Chronic Myocardial Infarction
Author(s) -
Yihua Liu,
Xiaoxi Yang,
Pablo Maureira,
Aude Falanga,
Vanessa Marie,
Guillaume Gauchotte,
Sylvain Poussier,
Frédérique Groubatch,
Pierre-Yves Marie,
Nguyen Tran
Publication year - 2017
Publication title -
cellular physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.486
H-Index - 87
eISSN - 1421-9778
pISSN - 1015-8987
DOI - 10.1159/000485406
Subject(s) - mesenchymal stem cell , medicine , bone marrow , cell therapy , paracrine signalling , in vivo , cytokine , pathology , neovascularization , hypoxia (environmental) , fibrosis , viability assay , angiogenesis , immunology , cancer research , cell , stem cell , biology , microbiology and biotechnology , chemistry , receptor , genetics , organic chemistry , oxygen
The mismatch between traditional in vitro cell culture conditions and targeted chronic hypoxic myocardial tissue could potentially hamper the therapeutic effects of implanted bone marrow mesenchymal stem cells (BMSCs). This study sought to address (i) the extent of change to BMSC biological characteristics in different in vitro culture conditions and (ii) the effectiveness of permanent hypoxic culture for cell therapy in treating chronic myocardial infarction (MI) in rats.