Open AccessEvaluation of the Expression of Genes Associated with Inflammation and Apoptosis in Androgenetic Alopecia by Targeted RNA-Seq
Author(s) -
Lizeth Martínez-Jacobo,
Claudia I Ancer-Arellano,
Rocío OrtizLópez,
Mauricio SalinasSantander,
César Daniel Villarreal-Villarreal,
Jesús ÁncerRodríguez,
Bianka Dianey Camacho-Zamora,
Viviana Zomosa-Signoret,
Carlos E. Medina-De la Garza,
Jorge OcampoCandiani,
Augusto Rojas-Martínez
Publication year - 2017
Publication title -
skin appendage disorders
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.773
H-Index - 13
eISSN - 2296-9195
pISSN - 2296-9160
DOI - 10.1159/000484530
Subject(s) - rna seq , inflammation , gene , apoptosis , gene expression , cancer research , rna , expression (computer science) , biology , transcriptome , computational biology , microbiology and biotechnology , bioinformatics , genetics , immunology , computer science , programming language
Androgenetic alopecia (AGA) or male pattern baldness is the most common form of hair loss in humans. Despite being a very frequent dermatological entity, molecular pathophysiology remains unclear. Several authors relate the presentation of AGA with a premature apoptotic process during the anagen phase and with an inflammatory microenvironment in the hair follicle. We evaluated a panel of 30 genes associated with inflammation and apoptosis in 5 AGA patients by targeted RNA-Seq. WNT7A gene was highly expressed in patients in stages 3V to 5 on the Hamilton-Norwood scale compared to patients with 5A stage. CASP7 and TNF genes were overexpressed in stages 3V and 4 compared to stages 5 and 5A. Overexpression of these genes detected only at early stages of AGA proves the role of WNT pathway, apoptosis, and inflammation in the development of this disorder.