Antagomirs Targeting MicroRNA-134 Increase Limk1 Levels After Experimental Seizures in Vitro and in Vivo | Zendy

Jiahang Sun | Zendy; Xiaoying Gao | Zendy; Dawei Meng | Zendy; Yang Xu | Zendy; Xichun Wang | Zendy; Xin Gu | Zendy; Mian Guo | Zendy; Xiaodong Shao | Zendy; Hongwen Yan | Zendy; Chuanlu Jiang | Zendy; Yongri Zheng | Zendy

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Antagomirs Targeting MicroRNA-134 Increase Limk1 Levels After Experimental Seizures in Vitro and in Vivo

Author(s) -

Jiahang Sun,

Xiaoying Gao,

Dawei Meng,

Yang Xu,

Xichun Wang,

Xin Gu,

Mian Guo,

Xiaodong Shao,

Hongwen Yan,

Chuanlu Jiang,

Yongri Zheng

Publication year - 2017

Publication title -

cellular physiology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.486

H-Index - 87

eISSN - 1421-9778

pISSN - 1015-8987

DOI - 10.1159/000480647

Subject(s) - neuroprotection , hippocampal formation , status epilepticus , hippocampus , microrna , reporter gene , microbiology and biotechnology , pilocarpine , pharmacology , chemistry , biology , epilepsy , neuroscience , gene expression , biochemistry , gene

Background: MiR-134 is enriched in dendrites of hippocampal neurons and plays crucial roles in the progress of epilepsy. The present study aims to investigate the effects of antagomirs targeting miroRNA-134 (Ant-134) on limk1 expression and the binding of miR-134 and limk1 in experimental seizure. Methods: Status epilepticus (SE) rat model was established by lithium chloride-pilocarpine injection and was treated with Ant-134 by intracerebroventricular injection. Low Mg2+-exposed primary neurons were used as an in vitro model of SE. The expression of miR-134 was determined using real-time PCR. Protein expressions of limk1 and cofilin were determined by Western blotting. Luciferase reporter assay was used to examine the binding between miR-134 and limk1 3’-untranslated region. Results: The expression of miR-134 was markedly enhanced in hippocampus of the SE rats and low Mg2+-exposed neurons. Ant-134 increased the expression of limk1 and reduced the expression of cofilin in the SE hippocampus and Low Mg2+-exposed neurons. In addition, luciferase reporter assay confirmed that miR-134 bound limk1 3’-UTR. MiR-134 overexpression inhibited limk1 mRNA and protein expressions in neurons. Conclusion: Blockage of miR-134 upregulates limk1 expression and downregulated cofilin expression in hippocampus of the SE rats. This mechanism may contribute to the neuroprotective effects of Ant-134

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