Open AccessSyncytia Induction by Clinical Isolates of Human Respiratory Syncytial Virus A
Author(s) -
Talita Bianca Gagliardi,
Miriã Ferreira Criado,
José Luiz Proença-Módena,
Ariane Mirela Saranzo,
Marisa Akiko Iwamoto,
Flávia Escremim de Paula,
Ricardo Cardoso,
Luana Sela Delcaro,
Maria Lúcia Silva,
A.A. Câmara,
Eurico Arruda
Publication year - 2017
Publication title -
intervirology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.641
H-Index - 61
eISSN - 1423-0100
pISSN - 0300-5526
DOI - 10.1159/000480014
Subject(s) - syncytium , biology , virus , virology , cytopathic effect , messenger rna , microbiology and biotechnology , in vitro , gene , genetics
Syncytia formation is the hallmark of the cytopathic effect caused by human respiratory syncytial virus (HRSV), which is the most important viral respiratory pathogen in children. This article reports methodological improvements in primary HRSV isolation and the importance of syncytia formation and mRNA levels of F protein for the progeny yield, using clinical isolates of HRSV.