Open AccessCollectin-11 Is an Important Modulator of Retinal Pigment Epithelial Cell Phagocytosis and Cytokine Production
Author(s) -
Xia Dong,
Weiju Wu,
Liang Ma,
Chengfei Liu,
Mohajeet Bhuckory,
Liping Wang,
Emeline F. Nandrot,
Heping Xu,
Ké Li,
Yizhi Liu,
Wuding Zhou
Publication year - 2017
Publication title -
journal of innate immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.078
H-Index - 64
eISSN - 1662-8128
pISSN - 1662-811X
DOI - 10.1159/000478042
Subject(s) - microbiology and biotechnology , phagocytosis , collectin , calreticulin , cytokine , biology , downregulation and upregulation , retinal , retinal pigment epithelium , retina , receptor , innate immune system , immunology , biochemistry , neuroscience , gene , endoplasmic reticulum
In this paper, we report previously unknown roles for collectin-11 (CL-11, a soluble C-type lectin) in modulating the retinal pigment epithelial (RPE) cell functions of phagocytosis and cytokine production. We found that CL-11 and its carbohydrate ligand are expressed in both the murine and human neural retina; these resemble each other in terms of RPE and photoreceptor cells. Functional analysis of murine RPE cells showed that CL-11 facilitates the opsonophagocytosis of photoreceptor outer segments and apoptotic cells, and also upregulates IL-10 production. Mechanistic analysis revealed that calreticulin on the RPE cells is required for CL-11-mediated opsonophagocytosis whereas signal-regulatory protein α and mannosyl residues on the cells are involved in the CL-11-mediated upregulation of IL-10 production. This study is the first to demonstrate the role of CL-11 and the molecular mechanisms involved in modulating RPE cell phagocytosis and cytokine production. It provides a new insight into retinal health and disease and has implications for other phagocytic cells.