Open AccessPam3CSK4 Induces MMP-9 Expression in Human Monocytic THP-1 Cells
Author(s) -
Fatema Al-Rashed,
Shihab Kochumon,
Safa Usmani,
Sardar Sindhu,
Rasheed Ahmad
Publication year - 2017
Publication title -
cellular physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.486
H-Index - 87
eISSN - 1421-9778
pISSN - 1015-8987
DOI - 10.1159/000475298
Subject(s) - thp1 cell line , mapk/erk pathway , tlr2 , signal transduction , p38 mitogen activated protein kinases , microbiology and biotechnology , cytokine , tlr4 , biology , cell culture , immunology , genetics
Matrix metalloproteinase (MMP)-9 is known to degrade the extracellular matrix and increased MMP-9 levels are related with the pathogenesis of many inflammatory conditions including obesity. Pam3CSK4 is a synthetic triacylated lipopeptide (LP) which is a potent activator of immune cells and induces cytokine production. However, it is unclear whether Pam3CSK4 is able to induce MMP-9 expression in monocytic cells. We, therefore, determined MMP-9 production by Pam3CSK4-treated THP-1 cells and also investigated the signal transduction pathway(s) involved.