Open AccessRebeccamycin Attenuates TNF-α-Induced Intestinal Epithelial Barrier Dysfunction by Inhibiting Myosin Light Chain Kinase Production
Author(s) -
Akihiro Watari,
Yuta Sakamoto,
Kota Hisaie,
Kazuki Iwamoto,
Miho Fueta,
Kiyohito Yagi,
Masuo Kondoh
Publication year - 2017
Publication title -
cellular physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.486
H-Index - 87
eISSN - 1421-9778
pISSN - 1015-8987
DOI - 10.1159/000472367
Subject(s) - myosin light chain kinase , barrier function , occludin , paracellular transport , tight junction , microbiology and biotechnology , proinflammatory cytokine , chemistry , biology , phosphorylation , biochemistry , immunology , inflammation , permeability (electromagnetism) , membrane
Although proinflammatory cytokine-induced disruption of intestinal epithelial barrier integrity is associated with intestinal inflammatory disease, effective treatment for barrier dysfunction is lacking. Previously, we demonstrated that rebeccamycin alleviates epithelial barrier dysfunction induced by inflammatory cytokines in Caco-2 cell monolayers; however, the underlying mechanism remained unclear. Here, we investigated the mechanism by which rebeccamycin protects the epithelial barrier function of Caco-2 cells exposed to TNF-α.