Open AccessImpact of miR-208 and its Target Gene Nemo-Like Kinase on the Protective Effect of Ginsenoside Rb1 in Hypoxia/Ischemia Injuried Cardiomyocytes
Author(s) -
Yan Xu,
Jianxun Liu,
Hongjin Wu,
Yuna Liu,
Sidao Zheng,
Chengying Zhang,
Chao Yang
Publication year - 2016
Publication title -
cellular physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.486
H-Index - 87
eISSN - 1421-9778
pISSN - 1015-8987
DOI - 10.1159/000447825
Subject(s) - mtt assay , hypoxia (environmental) , flow cytometry , apoptosis , viability assay , blot , transfection , luciferase , kinase , ischemia , ginseng , pharmacology , ginsenoside , reporter gene , microbiology and biotechnology , medicine , chemistry , gene expression , gene , biology , biochemistry , pathology , alternative medicine , organic chemistry , oxygen
Ginsenoside Rb1 (GS-Rb1) is one of the most important active pharmacological extracts of the Traditional Chinese Medicine ginseng, with extensive evidence of its cardioprotective properties. Mir-208 has been shown to act as a biomarker of acute myocardial infarction in vivo studies including man. However the impact of miR-208 on the protective effect of GS-Rb1 in hypoxia/ischemia injured cardiomyocytes remains unclear. The current study aims to investigate the target gene of miR-208 and the impact on the protective effect of GS-Rb1 in hypoxia/ischemia (H/I) injuried cardiomyocytes.