Open AccessClinical Usefulness of a One-Tube Nested Reverse Transcription Quantitative Polymerase Chain Reaction Assay for Evaluating Human Epidermal Growth Factor Receptor 2 mRNA Overexpression in Formalin-Fixed and Paraffin-Embedded Breast Cancer Tissue Samples
Author(s) -
Hye Young Wang,
Sung Gwe Ahn,
Sunyoung Park,
Seungil Kim,
Hyeyoung Lee
Publication year - 2016
Publication title -
pathobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 53
eISSN - 1423-0291
pISSN - 1015-2008
DOI - 10.1159/000447301
Subject(s) - immunohistochemistry , biology , reverse transcription polymerase chain reaction , microbiology and biotechnology , breast cancer , real time polymerase chain reaction , epidermal growth factor receptor , human epidermal growth factor receptor 2 , polymerase chain reaction , messenger rna , reverse transcriptase , epidermal growth factor , fluorescence in situ hybridization , in situ hybridization , pathology , cancer , cancer research , receptor , gene , medicine , immunology , genetics , chromosome
Currently, the two main methods used to analyze human epidermal growth factor receptor 2 (HER2) amplification or overexpression have a limited accuracy and high costs. These limitations can be overcome by the development of complementary quantitative methods.