Clinical Usefulness of a One-Tube Nested Reverse Transcription Quantitative Polymerase Chain Reaction Assay for Evaluating Human Epidermal Growth Factor Receptor 2 mRNA Overexpression in Formalin-Fixed and Paraffin-Embedded Breast Cancer Tissue Samples | Zendy

Hye-young Wang | Zendy; Sungwoo Ahn | Zendy; Sunyoung Park | Zendy; Seungil Kim | Zendy; Hyeyoung Lee | Zendy

Open Access

Clinical Usefulness of a One-Tube Nested Reverse Transcription Quantitative Polymerase Chain Reaction Assay for Evaluating Human Epidermal Growth Factor Receptor 2 mRNA Overexpression in Formalin-Fixed and Paraffin-Embedded Breast Cancer Tissue Samples

Author(s) -

Hye-young Wang,

Sungwoo Ahn,

Sunyoung Park,

Seungil Kim,

Hyeyoung Lee

Publication year - 2016

Publication title -

pathobiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.941

H-Index - 53

eISSN - 1423-0291

pISSN - 1015-2008

DOI - 10.1159/000447301

Subject(s) - immunohistochemistry , biology , reverse transcription polymerase chain reaction , microbiology and biotechnology , breast cancer , real time polymerase chain reaction , epidermal growth factor receptor , human epidermal growth factor receptor 2 , polymerase chain reaction , messenger rna , reverse transcriptase , epidermal growth factor , fluorescence in situ hybridization , in situ hybridization , pathology , cancer , cancer research , receptor , gene , medicine , immunology , genetics , chromosome

Currently, the two main methods used to analyze human epidermal growth factor receptor 2 (HER2) amplification or overexpression have a limited accuracy and high costs. These limitations can be overcome by the development of complementary quantitative methods.

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