Open AccessNocodazole Induced Suicidal Death of Human Erythrocytes
Author(s) -
Elena Signoretto,
Sabina Honisch,
Marilena Briglia,
Caterina Faggio,
Michela Castagna,
Florian Läng
Publication year - 2016
Publication title -
cellular physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.486
H-Index - 87
eISSN - 1421-9778
pISSN - 1015-8987
DOI - 10.1159/000438638
Subject(s) - nocodazole , apoptosis , phosphatidylserine , ceramide , microbiology and biotechnology , annexin , programmed cell death , biology , oxidative stress , chemistry , flow cytometry , reactive oxygen species , biophysics , cell , biochemistry , phospholipid , cytoskeleton , membrane
The microtubule assembly inhibitor nocodazole has been shown to trigger caspase-independent mitotic death and caspase dependent apoptosis. Similar to apoptosis of nucleated cells, erythrocytes may undergo eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the erythrocyte surface. Stimulators of eryptosis include increase of cytosolic Ca2+ activity ([Ca2+]i), oxidative stress and ceramide. The present study explored, whether and how nocodazole induces eryptosis.