Open AccessThe Effects of AS160 Modulation on Fatty Acid Transporters Expression and Lipid Profile in L6 Myotubes
Author(s) -
Agnieszka Mikłosz,
Bartłomiej Łukaszuk,
Małgorzata Żendzian-Piotrowska,
Krzysztof Kurek,
Adrian Chabowski
Publication year - 2016
Publication title -
cellular physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.486
H-Index - 87
eISSN - 1421-9778
pISSN - 1015-8987
DOI - 10.1159/000438628
Subject(s) - myogenesis , transporter , chemistry , microbiology and biotechnology , modulation (music) , fatty acid , biochemistry , biology , in vitro , gene , philosophy , aesthetics
AS160 is a key intracellular regulator of energy utilization in cells. It was shown to regulate GLUT4 translocation from intracellular depots to the plasma membrane, with subsequent changes in facilitated glucose uptake into the skeletal muscles. Similarly, also free fatty acids (FFAs) transmembrane transport seems to be largely protein-mediated. Therefore, the objective of this study was to examine the effects of moderate AS160 depletion (-82% mRNA, -25% of protein content) on the expression of fatty acid transporters and subsequent changes in lipid profile in L6 myotubes.