Open AccessTissue Distribution and Regulation of the Small Sar1b GTPase in Mice
Author(s) -
Valérie Marcil,
Ernest G. Seidman,
Daniel Sinnett,
Rocío Sánchez,
Schohraya Spahis,
Alain Théophile Sané,
Émile Lévy
Publication year - 2014
Publication title -
cellular physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.486
H-Index - 87
eISSN - 1421-9778
pISSN - 1015-8987
DOI - 10.1159/000362960
Subject(s) - biology , adipose tissue , endocrinology , medicine , skeletal muscle , genetically modified mouse , gene expression , transgene , copii , endoplasmic reticulum , microbiology and biotechnology , golgi apparatus , gene , secretory pathway , genetics
Sar1b GTPase (Sar1b) represents an obligatory component of COPII vesicles that bud from the endoplasmic reticulum to transport proteins to the Golgi apparatus. Its genetic mutations lead to a severe disorder known as chylomicron retention disease. Despite growing knowledge on Sar1b, little is known about it tissue distribution and regulation, which constitute the aims of the present study. We aimed to determine the Sar1b tissue distribution and modulation by a high-fat diet and gene forcing using transgenic mice in comparison to wild-type mice. Methods : The expression pattern of Sar1b was studied in different organs of wild-type and Sar1b transgenic mice by qRT-PCR and Western blot. The effect of transgenesis and insulin resistance induced by a 12-week high-fat diet on Sar1b gene expression was also assessed by qRT-PCR.