Open AccessA FRET-Based Approach for Quantitative Evaluation of Forskolin-Induced Pendrin Trafficking at the Plasma Membrane in Bronchial NCI H292 Cells
Author(s) -
Grazia Tamma,
Marianna Ranieri,
Silvia Dossena,
Annarita Di Mise,
Charity Nofziger,
María Svelto,
Markus Paulmichl,
Giovanna Valenti
Publication year - 2013
Publication title -
cellular physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.486
H-Index - 87
eISSN - 1421-9778
pISSN - 1015-8987
DOI - 10.1159/000356639
Subject(s) - pendrin , chemistry , microbiology and biotechnology , förster resonance energy transfer , context (archaeology) , biophysics , biology , biochemistry , transporter , gene , paleontology , physics , quantum mechanics , fluorescence
Human pendrin (SLC26A4, PDS) is an integral membrane protein acting as an electroneutral anion exchanger. Loss of function mutations in pendrin protein cause Pendred syndrome, a disorder characterized by sensorineural deafness and a partial iodide organification defect that may lead to thyroid goiter. Additionally, pendrin up-regulation could play a role in the pathogenesis of several diseases including bronchial asthma and chronic obstructive pulmonary disease (COPD). Therefore, monitoring the plasma membrane abundance and trafficking of pendrin in the context of a living cell is crucially important.