A FRET-Based Approach for Quantitative Evaluation of Forskolin-Induced Pendrin Trafficking at the Plasma Membrane in Bronchial NCI H292 Cells | Zendy

Grazia Tamma | Zendy; Marianna Ranieri | Zendy; Silvia Dossena | Zendy; Annarita Di Mise | Zendy; Charity Nofziger | Zendy; Maria Svelto | Zendy; Markus Paulmichl | Zendy; Giovanna Valenti | Zendy

Open Access

A FRET-Based Approach for Quantitative Evaluation of Forskolin-Induced Pendrin Trafficking at the Plasma Membrane in Bronchial NCI H292 Cells

Author(s) -

Grazia Tamma,

Marianna Ranieri,

Silvia Dossena,

Annarita Di Mise,

Charity Nofziger,

Maria Svelto,

Markus Paulmichl,

Giovanna Valenti

Publication year - 2013

Publication title -

cellular physiology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.486

H-Index - 87

eISSN - 1421-9778

pISSN - 1015-8987

DOI - 10.1159/000356639

Subject(s) - pendrin , chemistry , microbiology and biotechnology , förster resonance energy transfer , context (archaeology) , biophysics , biology , biochemistry , transporter , gene , paleontology , physics , quantum mechanics , fluorescence

Human pendrin (SLC26A4, PDS) is an integral membrane protein acting as an electroneutral anion exchanger. Loss of function mutations in pendrin protein cause Pendred syndrome, a disorder characterized by sensorineural deafness and a partial iodide organification defect that may lead to thyroid goiter. Additionally, pendrin up-regulation could play a role in the pathogenesis of several diseases including bronchial asthma and chronic obstructive pulmonary disease (COPD). Therefore, monitoring the plasma membrane abundance and trafficking of pendrin in the context of a living cell is crucially important.

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