Open AccessNuclear Transport of Wilms′ Tumour Protein Wt1 Involves Importins α and β
Author(s) -
Reinhard Depping,
Susanne Schindler,
C. A. Jacobi,
Karin M. Kirschner,
Holger Scholz
Publication year - 2012
Publication title -
cellular physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.486
H-Index - 87
eISSN - 1421-9778
pISSN - 1015-8987
DOI - 10.1159/000337603
Subject(s) - importin , nuclear transport , nuclear localization sequence , zinc finger , nls , biology , immunoprecipitation , microbiology and biotechnology , nuclear protein , cell nucleus , transcription factor , cytoplasm , cancer research , gene , genetics
Wilms' tumour protein, Wt1, is a zinc finger molecule, which is required for normal embryonic development. Mutations of the WT1 gene can give rise to childhood cancer of the kidneys. Different Wt1 isoforms exist, which function either as transcription factors or have a presumed role in mRNA processing. Previous studies suggested that Wt1 undergoes nucleocytoplasmic shuttling, and cytoplasmic Wt1 was higher in malignant than in normal cells. The aim of this study was to analyse the molecular pathways along which Wt1 shuttles between the cytoplasm and nucleus.