Open AccessStabilisation and Knockdown of HIF - Two Distinct Ways Comparably Important in Radiotherapy
Author(s) -
Mareike Ströfer,
Wolfgang Jelkmann,
Eric Metzen,
Ulf Brockmeier,
Jürgen Dunst,
Reinhard Depping
Publication year - 2011
Publication title -
cellular physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.486
H-Index - 87
eISSN - 1421-9778
pISSN - 1015-8987
DOI - 10.1159/000335794
Subject(s) - gene knockdown , radioresistance , radiosensitivity , clonogenic assay , hypoxia (environmental) , cancer research , radiation therapy , radiation sensitivity , oxygen enhancement ratio , cancer cell , biology , ionizing radiation , hypoxia inducible factors , cancer , cell , apoptosis , chemistry , medicine , oxygen , gene , biochemistry , irradiation , physics , organic chemistry , nuclear physics
Radiotherapy is one of the most widely used treatments for cancer. The benefit of radiation is known to be negatively affected by tumor hypoxia and the expression of hypoxia-inducible factors (HIF), respectively. HIF-1α/ β and HIF-2α/ β are transcriptional activators of oxygen-regulated genes. The aim of the study was to examine cell type-specific effects of HIF-1α and -2α knockdown or oxygen-independent HIF-stabilisation on radiosensitivity.