Open AccessBaicalein Suppresses the SOS Response System of <i>Staphylococcus Aureus</i> Induced by Ciprofloxacin
Author(s) -
Qing Peng,
Shuqin Zhou,
Fen Yao,
Bing Hou,
Yuanchun Huang,
Dexing Hua,
Yanshan Zheng,
Qian Yuanshu
Publication year - 2011
Publication title -
cellular physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.486
H-Index - 87
eISSN - 1421-9778
pISSN - 1015-8987
DOI - 10.1159/000335791
Subject(s) - baicalein , staphylococcus aureus , sos response , reactive oxygen species , microbiology and biotechnology , antibiotics , chemistry , ciprofloxacin , repressor lexa , pharmacology , bacteria , biology , escherichia coli , biochemistry , gene , gene expression , genetics , repressor
Numerous antibiotics can induce an SOS repair system in bacteria that leads to antibiotic-resistant mutation of the bacterium. Therefore, searching for drugs that can prevent the SOS response and thus improve the long-term viability of some antibiotics is important. In this study, we aimed to detect the suppressive effects of baicalein on the SOS system and rifampin-resistant mutation in Staphylococcus aureus. We determined the reactive oxygen species (ROS) formation and intracellular ATP level in S. aureus with baicalein treatment to investigate the mechanisms involved in its effects on the SOS system. The results showed that baicalein was a potent inhibitor of the expression of the SOS genes RecA, LexA and SACOL1400. The rifampin-resistant mutation rate of S. aureus induced by ciprofloxacin was significantly reduced after treatment with baicalein. Treatment with baicalein led to a significant decrease in intracellular reactive oxygen species (ROS) formation and ATP level. Our findings indicate that baicalein may be an SOS-response inhibitor in S. aureus through inhibiting ROS formation and ATP production and may be used to prevent excessive mutation induced by antibiotics.