Open AccessHyperhomocysteinemia is Associated with Decreased Erythropoietin Expression in Rats
Author(s) -
Almut Grenz,
Marina Hermes,
Pascal Hammel,
Jan B. Roll,
Hartmut Oßwald,
Doris Kloor
Publication year - 2010
Publication title -
cellular physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.486
H-Index - 87
eISSN - 1421-9778
pISSN - 1015-8987
DOI - 10.1159/000320568
Subject(s) - erythropoietin , hyperhomocysteinemia , medicine , endocrinology , homocysteine , methyltransferase , in vivo , chemistry , kidney , transmethylation , methylation , biology , biochemistry , microbiology and biotechnology , gene
Elevated plasma homocysteine (Hcy) levels have been identified as a pathogenic factor causing a variety of pathological changes in different cells and tissues. In vertebrates, Hcy is produced solely from S-adenosylhomocysteine (AdoHcy) through the catalysis of AdoHcy-hydrolase. The direction of AdoHcy-hydrolase activity is determined by its cytosolic substrate concentrations, thereby controlling intracellular AdoHcy levels. Most S-adenosylmethionine (AdoMet)-dependent methyltransferases are regulated in vivo by the ratio of AdoMet/AdoHcy, which is termed "methylation potential" (MP). To test whether high rates of erythropoietin (EPO) expression is reduced by a low MP in vivo we choosed the model of increased EPO production following carbon monoxide (CO) exposure in rats in which high transcriptional activity is responsible for renal EPO production.