Open AccessIntegrin-α<sub>5</sub> Mediates Epidermal Growth Factor-Induced Retinal Pigment Epithelial Cell Proliferation and Migration
Author(s) -
Чен Жен,
Changzheng Chen,
Wenrong Gong,
Junping Li,
Yiqiao Xing
Publication year - 2010
Publication title -
pathobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 53
eISSN - 1423-0291
pISSN - 1015-2008
DOI - 10.1159/000278290
Subject(s) - proliferative vitreoretinopathy , integrin , cell migration , cell growth , epidermal growth factor , microbiology and biotechnology , western blot , biology , retinal pigment epithelium , flow cytometry , cell culture , chemistry , cell , retinal , biochemistry , retinal detachment , genetics , gene
Proliferation and migration of retinal pigment epithelial (RPE) cells play a crucial role in proliferative vitreoretinopathy (PVR)-related pathology. Cytokines, including EGF, can result in RPE cell activation and cause PVR. In this study, integrin-alpha(5) expression was first studied in PVR membranes by immunofluorescence. Then the effect of EGF on integrin-alpha(5) expression was determined by flow cytometry, Western blot analysis and the reverse-transcription polymerase chain reaction (RT-PCR) in the ARPE-19 cell line. Proliferation and migration of ARPE-19 cells were measured by the methylthiazolyldiphenyl-tetrazolium bromide and Boyden chamber assays. We found that a higher level integrin-alpha(5) was present at the RPE cell surface in PVR compared with normal retina. EGF could dose dependently increase integrin-alpha(5) mRNA and protein levels in vitro. EGF promoted ARPE-19 cell proliferation and migration. Neutralizing integrin-alpha(5) by specific anti-integrin-alpha(5) antibody abolished most of the effects of EGF. The study provided evidence that EGF might influence PVR by promoting integrin-alpha(5) expression and subsequent proliferation and migration of RPE.