Open AccessDetection of Truncated Circular DNA Species in <i>Escherichia coli </i>with a PCV2-Containing Plasmid with a Single PCV2 Origin of Replication
Author(s) -
Jan Van Doorsselaere,
David Lefebvre,
Hans Nauwynck,
Peter Delputte
Publication year - 2009
Publication title -
intervirology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.641
H-Index - 61
eISSN - 1423-0100
pISSN - 0300-5526
DOI - 10.1159/000264203
Subject(s) - porcine circovirus , biology , plasmid , microbiology and biotechnology , restriction enzyme , escherichia coli , genome , dna , virology , genetics , virus , gene
Porcine circovirus type 2 (PCV2) is a small circular single-stranded DNA virus that causes postweaning multisystemic wasting syndrome in pigs. Cloning of the PCV2 genome in a plasmid allows the construction of infectious clones. Our objective was to clone single PCV2 genomes from an isolate containing a mixture of strains, in a plasmid in order to obtain pure PCV2 strains.