z-logo
open-access-imgOpen Access
Detection of Truncated Circular DNA Species in <i>Escherichia coli </i>with a PCV2-Containing Plasmid with a Single PCV2 Origin of Replication
Author(s) -
Jan Van Doorsselaere,
David Lefebvre,
Hans Nauwynck,
Peter Delputte
Publication year - 2009
Publication title -
intervirology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.641
H-Index - 61
eISSN - 1423-0100
pISSN - 0300-5526
DOI - 10.1159/000264203
Subject(s) - porcine circovirus , biology , plasmid , microbiology and biotechnology , restriction enzyme , escherichia coli , genome , dna , virology , genetics , virus , gene
Porcine circovirus type 2 (PCV2) is a small circular single-stranded DNA virus that causes postweaning multisystemic wasting syndrome in pigs. Cloning of the PCV2 genome in a plasmid allows the construction of infectious clones. Our objective was to clone single PCV2 genomes from an isolate containing a mixture of strains, in a plasmid in order to obtain pure PCV2 strains.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here