Open AccessChanges in Human Endothelial Cell Energy Metabolic Capacities during in vitro Cultivation. The Role of “Aerobic Glycolysis” and Proliferation
Author(s) -
Kirsten Peters,
Günter Kamp,
Ansgar Berz,
Ronald E. Unger,
Susanne Barth,
Achim Salamon,
Joachim Rychly,
Charles James Kirkpatrick
Publication year - 2009
Publication title -
cellular physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.486
H-Index - 87
eISSN - 1421-9778
pISSN - 1015-8987
DOI - 10.1159/000257490
Subject(s) - glycolysis , anaerobic glycolysis , umbilical vein , citric acid cycle , cell growth , metabolic pathway , biology , microbiology and biotechnology , biochemistry , enzyme , cell culture , cell , endothelial stem cell , in vitro , genetics
In this study the influence of cultivation and proliferation on energy metabolic characteristics of human umbilical vein endothelial cells (HUVEC) has been examined. The energy metabolic capacities of human endothelial cells freshly isolated from the umbilical vein were compared with those after cultivation for three passages and as subconfluent and confluent cultures.