Open AccessEffects of lnterleukin-4 or Stem Cell Factor on Mast Cell Mediator Release and Cytokine Gene Expression
Author(s) -
John W. Coleman,
Mark G. Buckley,
Alison Taylor,
Edwin M. Banks,
Cmm Williams,
Mark R. Holliday,
Jeffrey P. Thompson
Publication year - 1995
Publication title -
international archives of allergy and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 100
eISSN - 1423-0097
pISSN - 1018-2438
DOI - 10.1159/000236961
Subject(s) - stem cell factor , mast cell , interleukin 33 , cytokine , biology , interleukin 5 , ionomycin , microbiology and biotechnology , mediator , immunology , stem cell , interleukin , haematopoiesis , intracellular
We have investigated the capacity of interleukin (IL)-4 or stem cell factor (SCF) to induce direct mediator release from rodent peritoneal mast cells, and also to induce or regulate cytokine gene expression in the human HMC-1 mast cell line. SCF, but not IL-4, induced low levels of serotonin release from mouse or rat peritoneal mast cells; rat mast cells acquired enhanced responsiveness to SCF during culture. IL-4, but not SCF, enhanced ionomycin-induced transcription and secretion of several genes, including the cytokines IL-3, IL-4, granulocyte/macrophage-colony-stimulating factor, IL-8 and the receptor for IL-6 in the human HMC-1 mast cell line.