Open AccessEffect of Nitric Oxide Generators on Ischemia-Reperfusion Injury and Histamine Release in Isolated Perfused Guinea Pig Heart
Author(s) -
Emanuela Masini,
Francesco Gambassi,
S. Bianchi,
Laura Mugnai,
M. Lupini,
Alessandra Pistelli,
P. F. Mannaioni
Publication year - 1991
Publication title -
international archives of allergy and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 100
eISSN - 1423-0097
pISSN - 1018-2438
DOI - 10.1159/000235376
Subject(s) - histamine , nitric oxide , ligature , ischemia , calcium , lactate dehydrogenase , perfusion , mast cell , medicine , guinea pig , anesthesia , pharmacology , chemistry , immunology , biochemistry , enzyme
In an ischemia-reperfusion model obtained in isolated perfused guinea pig heart by means of a double ligature of the left anterior descending coronary artery, the reperfusion of the ischemic myocardium leads to a release of lactate dehydrogenase and histamine, related to a decrease in the microdensitometry of cardiac mast cells and to a tissue calcium overload. The perfusion of the heart with L -arginine and with nitric oxide donors significantly reduces the release of histamine, the loss of mast cell metachromasia and calcium overload. These effects were potentiated by superoxide dismutase.