Open AccessInfluence of NO Synthase Inhibitor L-NAME on Parasitemia and Survival of <i>Plasmodium berghei</i> Infected Mice
Author(s) -
Saisudha Koka,
Camelia Lang,
Olivier M. Niemoeller,
Krishna M. Boini,
Jan P. Nicolay,
Stephan M. Huber,
Florian Läng
Publication year - 2008
Publication title -
cellular physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.486
H-Index - 87
eISSN - 1421-9778
pISSN - 1015-8987
DOI - 10.1159/000129641
Subject(s) - parasitemia , plasmodium berghei , malaria , phosphatidylserine , plasmodium falciparum , biology , life span , pharmacology , immunology , biochemistry , phospholipid , evolutionary biology , membrane
Accelerated suicidal death or eryptosis of infected erythrocytes may delay development of parasitemia in malaria. Eryptosis is inhibited by nitric oxide (NO). The present study has been performed to explore, whether inhibition of NO synthase by L-NAME modifies the course of malaria. We show here that L-NAME (>or=10 microM) increased phosphatidylserine exposure of Plasmodium falciparum infected human erythrocytes, an effect significantly more marked than in noninfected human erythrocytes. We further show that parasitemia in Plasmodium berghei infected mice was significantly decreased (from 50% to 18% of circulating erythrocytes 20 days after infection) by addition of 1 mg/ml L-NAME to the drinking water. According to CFSE labelling L-NAME treatment accelerated the clearance of both, noninfected and infected, erythrocytes from circulating blood, but did not significantly extend the life span of infected animals. In conclusion, treatment with L-NAME shortens the life span of circulating erythrocytes and thus delays development of parasitemia during malaria.