Open AccessExpression and Localization of S-Adenosylhomocysteine-Hydrolase in the Rat Kidney Following Carbon Monoxide Induced Hypoxia
Author(s) -
Doris Kloor,
Marina Hermes,
Katrin Fink,
Heide Schmid,
Karin Klingel,
Andreas F. Mack,
Almut Grenz,
Hartmut Oßwald
Publication year - 2007
Publication title -
cellular physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.486
H-Index - 87
eISSN - 1421-9778
pISSN - 1015-8987
DOI - 10.1159/000099192
Subject(s) - transmethylation , kidney , hydrolase , adenosine , chemistry , hypoxia (environmental) , microbiology and biotechnology , biology , biochemistry , endocrinology , enzyme , methylation , oxygen , organic chemistry , gene
Tissue hypoxia induces a variety of functional changes including enhanced transcriptional activity associated with high transmethylation activity (e.g. mRNA cap methylation) in the nucleus. It is well known that the kidney responds to hypoxia with enhanced transcription of erythropoietin (EPO) in the interstitial cells. Since S-adenosylhomocysteine (AdoHcy)-hydrolase regulates most S-adenosylmethionine (AdoMet) dependent transmethylation reactions by hydrolyzing the potent feedback inhibitor AdoHcy to adenosine and homocysteine we studied the effect of hypoxia by carbon monoxide (CO) inhalation (1200 ppm) on AdoHcy-hydrolase gene expression and its localization in rat kidneys.