Open AccessMultiplex Immunofluorescence in Formalin-Fixed Paraffin-Embedded Tumor Tissue to Identify Single-Cell–Level PI3K Pathway Activation
Author(s) -
Konrad H. Stopsack,
Ying Huang,
Svitlana Tyekucheva,
Travis Gerke,
Clyde Bango,
Habiba Elfandy,
Michaela Bowden,
Kathryn L. Penney,
Thomas M. Roberts,
Giovanni Parmigiani,
Philip W. Kantoff,
Lorelei A. Mucci,
Massimo Loda
Publication year - 2020
Publication title -
clinical cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.427
H-Index - 324
eISSN - 1557-3265
pISSN - 1078-0432
DOI - 10.1158/1078-0432.ccr-20-2000
Subject(s) - pten , tissue microarray , pi3k/akt/mtor pathway , hazard ratio , prostate cancer , medicine , biochemical recurrence , multiplex , interquartile range , cancer research , pathology , immunofluorescence , cancer , oncology , confidence interval , biology , bioinformatics , signal transduction , immunology , prostatectomy , biochemistry , antibody
Identifying cancers with high PI3K pathway activity is critical for treatment selection and eligibility into clinical trials of PI3K inhibitors. Assessments of tumor signaling pathway activity need to consider intratumoral heterogeneity and multiple regulatory nodes.