Open AccessA Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii
Author(s) -
Mahyar Porbaran,
Hamed Tahmasebi,
Mohammad Reza Arabestani
Publication year - 2021
Publication title -
international journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 40
eISSN - 1687-9198
pISSN - 1687-918X
DOI - 10.1155/2021/5565537
Subject(s) - acinetobacter baumannii , microbiology and biotechnology , aztreonam , gene , biology , antibiotics , siderophore , polymerase chain reaction , drug resistance , gentamicin , antibiotic resistance , bacteria , genetics , imipenem , pseudomonas aeruginosa
Background The iron/siderophore uptake system (IUS) involved in the Acinetobacter baumannii pathogenicity. However, IUS's role in antibiotic resistance and the production of β -lactamase enzymes of A. baumannii are unclear. This study aimed to investigate the relationship between the production of β -lactamase enzymes and IUS regulatory genes in clinical isolates of A. baumannii . Methods. A. baumannii isolates were collected from clinical isolates using biochemical tests. The antibiotic resistance patterns and β -lactamase-producing strains were identified using the disk diffusion method (DDM). Also, IUS genes were detected by the polymerase chain reaction (PCR) method.Results Seventy-two (72) A. baumannii isolates were collected from a different clinical specimen. Gentamicin-resistant strains (43%) had the highest frequency, and aztreonam-resistant strains (12.5%) had the lowest frequency. Also, the distribution of AmpC and MBL producing isolates were 27.7% and 35%, respectively. Moreover, the frequencies of basD , bauA , pld , paaE , entA , feoB , hemO , and tonB genes were as follows: 12.5%, 15.2%, 11.1%, 15.2%, 19.4%, 16.6%, 23.6%, and 6.9%. Further, a strong correlation was observed between the abundance of β -lactamase-producing strains and IUS genes.Conclusions Based on our knowledge from this study, the association between β -lactamase production and IUS genes in A. baumannii plays an essential role in the emergence of drug-resistant strains.