Open AccessAntibacterial, Antibiofilm, and Antioxidant Activity of Polysaccharides Obtained from Fresh Sarcotesta of Ginkgo biloba: Bioactive Polysaccharide that Can Be Exploited as a Novel Biocontrol Agent
Author(s) -
Hong Jiang,
Zuxiang Luan,
Fan Zhang,
Xinliang Wu,
Ziheng Xu,
Tiezhong Zhou,
Hongjun Wang
Publication year - 2021
Publication title -
evidence-based complementary and alternative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.552
H-Index - 90
eISSN - 1741-4288
pISSN - 1741-427X
DOI - 10.1155/2021/5518403
Subject(s) - biofilm , polysaccharide , microbiology and biotechnology , dpph , abts , antioxidant , ginkgo biloba , biology , staphylococcus aureus , icad , mannose , antibacterial activity , hemolysis , chemistry , biochemistry , food science , bacteria , pharmacology , gene , genetics , immunology
Staphylococcus aureus ( S. aureus ) biofilm plays an important role in the persistence of chronic infection due to its resistance to antibiotics. Because of their functional diversity, active polysaccharide is increasingly being applied as a biocontrol agent to inhibit the formation of biofilm by pathogens. In this study, a new polysaccharide, GBSPII-1, isolated from the fresh sarcotesta of Ginkgo biloba L . ( G. biloba ) was characterized and its effect on antibiofilm formation of S. aureus was examined in vitro . High-Performance Liquid Chromatography (HPLC) analysis showed that GBSPII-1 is an acidic heteropolysaccharide composed of mannose, rhamnose, glucose, glucuronic acid, and galacturonic acid. GBSPII-1 demonstrated a molecular weight of 34 kDa and may affect the accumulation of polysaccharide intercellular adhesion (PIA) by inhibiting ica A, ica B, ica C, and ica D gene expression at subinhibitory concentrations. Under 10 g/L, GBSPII-1 showed an antioxidant effect on the inhibition rate of H 2 O 2 -induced erythrocyte hemolysis and the scavenging rate of DPPH radicals was 76.5 ± 0.5% and 89.2 ± 0.26%, respectively. The findings obtained in this study indicate that GBSPII-1 has antibacterial effect, is a possible source of natural antioxidants, and may be a potential biocontrol agent for the design of new therapeutic strategies for biofilm-related S. aureus infections.