Open AccessAsialoglycoprotein Receptor-Targeted Superparamagnetic Perfluorooctylbromide Nanoparticles
Author(s) -
Yang Li,
Chao Feng Yang,
Hui Zuo,
Ao Li,
Sushant Kumar Das,
Jin Yu
Publication year - 2021
Publication title -
contrast media and molecular imaging/contrast media and molecular imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.714
H-Index - 50
eISSN - 1555-4317
pISSN - 1555-4309
DOI - 10.1155/2021/5510071
Subject(s) - asialoglycoprotein receptor , in vivo , in vitro , chemistry , sonication , superparamagnetism , parenchyma , receptor , hepatocyte , microbiology and biotechnology , pathology , biochemistry , chromatography , medicine , biology , physics , magnetization , quantum mechanics , magnetic field
Background The decrease in asialoglycoprotein receptor (ASGPR) levels is observed in patients with chronic liver disease and liver tumor. The aim of our study was to develop ASGPR-targeted superparamagnetic perfluorooctylbromide nanoparticles (M-PFONP) and wonder whether this composite agent could target buffalo rat liver (BRL) cells in vitro and could improve R 2 ∗ value of the rat liver parenchyma after its injection in vivo.Methods GalPLL, a ligand of ASGPR, was synthesized by reductive amination. ASGPR-targeted M-PFOBNP was prepared by a film hydration method coupled with sonication. Several analytical methods were used to investigate the characterization and safety of the contrast agent in vitro. The in vivo MR T 2 ∗ mapping was performed to evaluate the enhancement effect in rat liver.Results The optimum concentration of Fe 3 O 4 nanoparticles inclusion in GalPLL/M-PFOBNP was about 52.79 µ g/mL, and the mean size was 285.6 ± 4.6 nm. The specificity of GalPLL/M-PFOBNP for ASGPR was confirmed by incubation experiment with fluorescence microscopy. The methyl thiazolyl tetrazolium (MTT) test showed that there was no significant difference in the optical density (OD) of cells incubated with all GalPLL/M-PFOBNP concentrations. Compared with M-PFOBNP, the increase in R 2 ∗ value of the rat liver parenchyma after GalPLL/M-PFOBNP injection was higher.Conclusions GalPLL/M-PFOBNP may potentially serve as a liver-targeted contrast agent for MR receptor imaging.