Open AccessInterfering Expression of Chimeric Transcript SEPT7P2-PSPH Promotes Cell Proliferation in Patients with Nasopharyngeal Carcinoma
Author(s) -
Jing Wang,
Guo Feng Xie,
Yuan He,
Ling Deng,
Ya Kang Long,
Xin Yang,
Jun Jiang,
Rui Gong,
Wen Jian Cen,
Zu Lu Ye,
Yi-Xin Zeng,
Hai Yun Wang,
Jian Yong Shao
Publication year - 2019
Publication title -
journal of oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.228
H-Index - 54
eISSN - 1687-8469
pISSN - 1687-8450
DOI - 10.1155/2019/1654724
Subject(s) - nasopharyngeal carcinoma , biology , rna splicing , fusion gene , sanger sequencing , gene , alternative splicing , cell culture , cancer research , fusion transcript , microbiology and biotechnology , genetics , messenger rna , rna , mutation , medicine , radiation therapy
Nasopharyngeal carcinoma (NPC) is a distinct type of head and neck cancer which is mostly prevalent in southern China. The development of NPC involves accumulation of multiple genetic changes. Chromosomal translocation is always thought to be accompanied with the fusion chimeric products. To data, the role of the fusion chimeric transcript remains obscure.Materials and Methods We performed RNA sequencing to detect the fusion genes in ten NPC tissues. Sanger sequencing and quantitative RT-PCR were used to measure the level of the fusion chimeric transcript in NPC tissues and cell lines. The functional experiments such as CCK8 assay, colony formation, and migration/invasion were conducted to analyze the role of this transcript in NPC in vitro .Results We demonstrated that the chimeric transcript SEPT7P2-PSPH was formed by trans-splicing of adjacent genes in the absence of chromosomal rearrangement and observed in both NPC patients and cell lines in parallel. Low-expression of the SEPT7P2-PSPH chimeric transcript induced the protein expression of PSPH and promoted cell proliferation, metastasis/invasion, and transforming ability in vitro .Conclusions Our findings indicate that the chimeric transcript SEPT7P2-PSPH is a product of trans-splicing of two adjacent genes and might be a tumor suppressor gene, potentially having the role of anticancer activity.