Open AccessIntragastric and Intranasal Administration ofLactobacillus paracaseiNCC2461 Modulates Allergic Airway Inflammation in Mice
Author(s) -
Céline Pellaton,
Sophie Nutten,
AnneChristine Thierry,
Caroline Boudousquié,
Nathalie Barbier,
Carine Blanchard,
Blaise Corthésy,
Annick Mercenier,
François Spertini
Publication year - 2012
Publication title -
international journal of inflammation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.106
H-Index - 33
eISSN - 2090-8040
pISSN - 2042-0099
DOI - 10.1155/2012/686739
Subject(s) - medicine , nasal administration , lactobacillus paracasei , inflammation , immunology , eotaxin , probiotic , chemokine , biology , bacteria , genetics
. Preclinical and clinical evidences for a role of oral probiotics in the management of allergic diseases are emerging. Aim . We aimed at testing the immunomodulatory effects of intranasal versus intragastric administration of Lactobacillus paracasei NCC2461 in a mouse model of allergic airway inflammation and the specificity of different probiotics by comparing L. paracasei NCC2461 to Lactobacillus plantarum NCC1107. Methods . L. paracasei NCC2461 or L. plantarum NCC1107 strains were administered either intragastrically (NCC2461) or intranasally (NCC2461 or NCC1107) to OVA-sensitized mice challenged with OVA aerosols. Inflammatory cell recruitment into BALF, eotaxin and IL-5 production in the lungs were measured. Results . Intranasal L. paracasei NCC2461 efficiently protected sensitized mice upon exposure to OVA aerosols in a dose-dependent manner as compared to control mice. Inflammatory cell number, eotaxin and IL-5 were significantly reduced in BALF. Intranasal supplementation of L. paracasei NCC2461 was more potent than intragastric application in limiting the allergic response and possibly linked to an increase in T regulatory cells in the lungs. Finally, intranasal L. plantarum NCC1107 reduced total and eosinophilic lung inflammation, but increased neutrophilia and macrophages infiltration. Conclusion . A concerted selection of intervention schedule, doses, and administration routes (intranasal versus intragastric) may markedly contribute to modulate airway inflammation in a probiotic strain-specific manner.