Open AccessLOH at 1p31 (ARHI) and Proliferation in Lymph Node-Negative Breast Cancer
Author(s) -
Emiel A. M. Janssen,
Irene Tveiterås Øvestad,
Ivar Skaland,
Håvard Søiland,
Einar Gudlaugsson,
Kjell H. Kjellevold,
Arne Nysted,
Jon-Arne Søreide,
Jan P. A. Baak
Publication year - 2009
Publication title -
analytical cellular pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 24
eISSN - 2210-7185
pISSN - 2210-7177
DOI - 10.1155/2009/304747
Subject(s) - breast cancer , oncology , medicine , lymph node , cancer , chemotherapy , mitotic index , immunohistochemistry , multivariate analysis , cancer research , biology , mitosis , microbiology and biotechnology
Background : The mitotic activity index (MAI) is a strong prognosticator in node-negative invasive breast cancer patients. Recently, a correlation between the MAI and specific chromosomal aberrations at chromosome 1p was described. Methods : Analysis of MAI, immunohistochemical staining patterns for proliferation-associated phosphohistone H3 (PPH3), phosphorylated ERK1/2, p21, cyclin E, Ki67 and cyclin D1 proteins; and prognosis in 158 adjuvant chemotherapy-treated T1-2N0M0 invasive breast cancer patients, analysis of LOH at 1p31 (including ARHI) using the dinucleotide repeats D1S207, D1S430 and D1S464 in 76 patients. Single and multivariate survival analysis was used to evaluate the importance of the various markers tested. Results : LOH at 1p31 did not correlate with MAI nor provide prognostic information. Phosphohistone H3 was the best prognosticator for patients in all age groups with 20 year distant metastasis free survival of distant metastases 93% vs. 72% respectively ( p = 0.004, HR = 4.5). In multivariate analysis, phosphohistone H3 < 13 vs. ≥13 exceeded the prognostic value of the mitotic activity index. Conclusions : LOH at 1p31 is common in breast cancer, and correlates with loss of proliferation-associated proteins, but not with MAI, PPH3 or prognosis. PPH3 is the best prognosticator in this study group of adjuvant chemotherapy-treated lymph node-negative breast cancer patients.