Open AccessDevelopmental shift to mitochondrial respiration for energetic support of sustained transmission during maturation at the calyx of Held
Author(s) -
Brendan Lujan,
Mahendra Singh,
Abhyudai Singh,
Robert Renden
Publication year - 2021
Publication title -
journal of neurophysiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.302
H-Index - 245
eISSN - 1522-1598
pISSN - 0022-3077
DOI - 10.1152/jn.00333.2021
Subject(s) - neurotransmission , synapse , respiration , glycolysis , oxidative phosphorylation , synaptic vesicle , mitochondrion , biology , neuroscience , bioenergetics , microbiology and biotechnology , transmission (telecommunications) , metabolism , vesicle , biochemistry , anatomy , computer science , telecommunications , receptor , membrane
We dissect the role of presynaptic glycolysis versus mitochondrial respiration in supporting high-frequency neurotransmission, by acutely blocking these ATP-generating pathways at a synapse tuned for high-frequency transmission. We find that massive energy expenditure is required to generate failure when only one pathway is inhibited. Action potential propagation is lost before impaired synaptic vesicle recycling. Synaptic transmission is exclusively dependent on OxPhos in mature synapses, indicating presynaptic glycolysis may be dispensable for ATP maintenance.