Open AccessAerobic exercise in humans mobilizes HSCs in an intensity-dependent manner
Author(s) -
Jeff Baker,
Joshua P. Nederveen,
Gianni Parise
Publication year - 2017
Publication title -
journal of applied physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.253
H-Index - 229
eISSN - 8750-7587
pISSN - 1522-1601
DOI - 10.1152/japplphysiol.00696.2016
Subject(s) - cd34 , haematopoiesis , peripheral blood mononuclear cell , medicine , mobilization , bone marrow , progenitor cell , hematocrit , immunology , stem cell , homing (biology) , endocrinology , biology , andrology , microbiology and biotechnology , biochemistry , history , archaeology , in vitro , ecology
Hematopoietic stem and progenitor cells are necessary to maintain, repair, and reconstitute the hematopoietic blood cell system. Mobilization of these cells from bone marrow to blood can be greatly increased under certain conditions, one such being exercise. The purpose of this study was to identify the importance of exercise intensity in hematopoietic mobilization, to better understand the mobilization kinetics postexercise, and to determine if exercise is capable of mobilizing several specific populations of hematopoietic cells that have clinical relevance in a transplant setting. Healthy individuals were exercised on a cycle ergometer at 70% of their peak work rate (WR peak ) until volitional fatigue and at 30% of their WR peak work matched to the 70% WR peak bout. Blood was collected before, immediately post, and 10, 30, and 60 min postexercise. Total blood cells, hematocrit, and mononuclear cells isolated by density gradient centrifugation were counted. Specific populations of hematopoietic stem cells were analyzed by flow cytometry. Mononuclear cells, CD34 + , CD34 + /CD38 - , CD34 + /CD110 + , CD3 - /CD16 + /CD56 + , CD11c + /CD123 - , and CD11c - /CD123 + cells per millilter of blood increased postexercise. Overall, the 70% WR peak exercise group showed greater mobilization immediately postexercise, while there was no observable increase in mobilization in the work matched 30% WR peak exercise group. Mobilization of specific populations of hematopoietic cells mirrored changes in the general mobilization of mononuclear cells, suggesting that exercise serves as a nonspecific mobilization stimulus. Evidently, higher intensity exercise is capable of mobilizing hematopoietic cells to a large extent and immediately postexercise is an ideal time point for their collection.