Open AccessSp1trans-activates the murine H+-K+-ATPase α2-subunit gene
Author(s) -
Zhiyuan Yu,
Mei Li,
Dongyu Zhang,
William Xu,
Bruce C. Kone
Publication year - 2009
Publication title -
europe pmc (pubmed central)
Language(s) - English
Resource type - Journals
eISSN - 1522-1466
DOI - 10.1152/ajprenal.00039.2009
Subject(s) - microbiology and biotechnology , chromatin immunoprecipitation , gene knockdown , promoter , biology , transcription factor , transfection , electrophoretic mobility shift assay , transcription (linguistics) , gene expression , response element , luciferase , transcriptional regulation , chromatin , gene , biochemistry , linguistics , philosophy
The H + -K + -ATPase α 2 (HKα2) gene of the renal collecting duct and distal colon plays a central role in potassium and acid-base homeostasis, yet its transcriptional control remains poorly characterized. We previously demonstrated that the proximal 177 bp of its 5′-flanking region confers basal transcriptional activity in murine inner medullary collecting duct (mIMCD3) cells and that NF-κB and CREB-1 bind this region to alter transcription. In the present study, we sought to determine whether the −144/−135 Sp element influences basal HKα2 gene transcription in these cells. Electrophoretic mobility shift and supershift assays using probes for −154/−127 revealed Sp1-containing DNA-protein complexes in nuclear extracts of mIMCD3 cells. Chromatin immunoprecipitation (ChIP) assays demonstrated that Sp1, but not Sp3, binds to this promoter region of the HKα2 gene in mIMCD3 cells in vivo. HKα2 minimal promoter-luciferase constructs with point mutations in the −144/−135 Sp element exhibited much lower activity than the wild-type promoter in transient transfection assays. Overexpression of Sp1, but not Sp3, trans-activated an HKα2 proximal promoter-luciferase construct in mIMCD3 cells as well as in SL2 insect cells, which lack Sp factors. Conversely, small interfering RNA knockdown of Sp1 inhibited endogenous HKα2 mRNA expression, and binding of Sp1 to chromatin associated with the proximal HKα2 promoter without altering the binding or regulatory influence of NF-κB p65 or CREB-1 on the proximal HKα2 promoter. We conclude that Sp1 plays an important and positive role in controlling basal HKα2 gene expression in mIMCD3 cells in vivo and in vitro.