Open AccessCYR61 (CCN1) overexpression induces lung injury in mice
Author(s) -
Serge Grazioli,
Sucheol Gil,
Dowon An,
Osamu Kajikawa,
Alex W. Farnand,
Josiah F. Hanson,
Timothy P. Birkland,
Peter C. Chen,
Jeremy S. Duffield,
Lynn M. Schnapp,
William A. Altemeier,
Gustavo Matute-Bello
Publication year - 2015
Publication title -
american journal of physiology. lung cellular and molecular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.892
H-Index - 163
eISSN - 1522-1504
pISSN - 1040-0605
DOI - 10.1152/ajplung.00190.2014
Subject(s) - cyr61 , ctgf , matricellular protein , bronchoalveolar lavage , downregulation and upregulation , pulmonary fibrosis , lung , myofibroblast , cancer research , fibrosis , medicine , pathology , biology , growth factor , extracellular matrix , gene , microbiology and biotechnology , receptor , biochemistry
Cysteine-rich protein-61 (CYR61), also known as connective tissue growth factor, CYR61, and nephroblastoma overexpressed gene 1 (CCN1), is a heparin-binding protein member of the CCN family of matricellular proteins. Gene expression profiles showed that Cyr61 is upregulated in human acute lung injury (ALI), but its functional role is unclear. We hypothesized that CYR61 contributes to ALI in mice. First, we demonstrated that CYR61 expression increases after bleomycin-induced lung injury. We then used adenovirus-mediated gene transfer to determine whether CYR61 overexpression in the lungs was sufficient to cause ALI. Mice instilled with CYR61 adenovirus showed greater weight loss, increased bronchoalveolar lavage total neutrophil counts, increased protein concentrations, and increased mortality compared with mice instilled with empty-vector adenovirus. Immunohistochemical studies in lungs from humans with idiopathic pulmonary fibrosis revealed CYR61 expression on the luminal membrane of alveolar epithelial cells in areas of injury. We conclude that CYR61 is upregulated in ALI and that CYR61 overexpression exacerbates ALI in mice.