Open AccessNFATc3 regulation of collagen V expression contributes to cellular immunity to collagen type V and hypoxic pulmonary hypertension
Author(s) -
Joshua R. Sheak,
David T. Jones,
Benjamin J. Lantz,
Levi Maston,
Danielle Vigil,
Thomas C. Resta,
Micaela M Resta,
Tamara Howard,
Nancy L. Kanagy,
Yan Guo,
Ewa JankowskaGan,
Jeremy A. Sullivan,
Rudolf Braun,
William J. Burlingham,
Laura V. González Bosc
Publication year - 2020
Publication title -
american journal of physiology. lung cellular and molecular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.892
H-Index - 163
eISSN - 1522-1504
pISSN - 1040-0605
DOI - 10.1152/ajplung.00184.2020
Subject(s) - immunostaining , immune system , pulmonary hypertension , extracellular matrix , microbiology and biotechnology , inflammation , in vivo , type iv collagen , autoimmunity , immunity , chemistry , immunology , biology , medicine , immunohistochemistry , laminin
Chronic hypoxia (CH)-induced pulmonary hypertension (PH) results, in part, from T helper-17 (T H 17) cell-mediated perivascular inflammation. However, the antigen(s) involved is unknown. Cellular immunity to collagen type V (col V) develops after ischemia-reperfusion injury during lung transplant and is mediated by naturally occurring (n)T H 17 cells. Col5a1 gene codifies for the α1-helix of col V, which is normally hidden from the immune system within type I collagen in the extracellular matrix. COL5A1 promoter analysis revealed nuclear factor of activated T cells, cytoplasmic 3 (NFATc3) binding sites. Therefore, we hypothesized that smooth muscle NFATc3 upregulates col V expression, leading to nT H 17 cell-mediated autoimmunity to col V in response to CH, representing an upstream mechanism in PH development. To test our hypothesis, we measured indexes of PH in inducible smooth muscle cell (SMC)-specific NFATc3 knockout (KO) mice exposed to either CH (380 mmHg) or normoxia and compared them with wild-type (WT) mice. KO mice did not develop PH. In addition, COL5A1 was one of the 1,792 genes differentially affected by both CH and SMC NFATc3 in isolated intrapulmonary arteries, which was confirmed by RT-PCR and immunostaining. Cellular immunity to col V was determined using a trans vivo delayed-type hypersensitivity assay (Tv-DTH). Tv-DTH response was evident only when splenocytes were used from control mice exposed to CH but not from KO mice, and mediated by nT H 17 cells. Our results suggest that SMC NFATc3 is important for CH-induced PH in adult mice, in part, by regulating the expression of the lung self-antigen COL5A1 protein contributing to col V-reactive nT H 17-mediated inflammation and hypertension.