Open AccessOpsin 3 and 4 mediate light-induced pulmonary vasorelaxation that is potentiated by G protein-coupled receptor kinase 2 inhibition
Author(s) -
Sebastian F. Barreto-Ortiz,
Daijiro Hori,
Yohei Nomura,
Yun Xing,
Haiyang Jiang,
Hwanmee Yong,
James Chen,
Sam Paek,
Deepesh Pandey,
Gautam Sikka,
Anil Bhatta,
Andrew G. Gillard,
Jochen Steppan,
Jae Hyung Kim,
Hideo Adachi,
Viachaslau Barodka,
Lewis H. Romer,
Steven S. An,
Larissa A. Shimoda,
Lakshmi Santhanam,
Dan E. Berkowitz
Publication year - 2018
Publication title -
american journal of physiology. lung cellular and molecular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.892
H-Index - 163
eISSN - 1522-1504
pISSN - 1040-0605
DOI - 10.1152/ajplung.00091.2017
Subject(s) - beta adrenergic receptor kinase , opsin , medicine , endocrinology , cgmp dependent protein kinase , myosin light chain kinase , biology , protein kinase a , chemistry , receptor , microbiology and biotechnology , g protein coupled receptor , kinase , rhodopsin , retinal , biochemistry , phosphorylation , mitogen activated protein kinase kinase
We recently demonstrated that blue light induces vasorelaxation in the systemic mouse circulation, a phenomenon mediated by the nonvisual G protein-coupled receptor melanopsin (Opsin 4; Opn4). Here we tested the hypothesis that nonvisual opsins mediate photorelaxation in the pulmonary circulation. We discovered Opsin 3 (Opn3), Opn4, and G protein-coupled receptor kinase 2 (GRK2) in rat pulmonary arteries (PAs) and in pulmonary arterial smooth muscle cells (PASMCs), where the opsins interact directly with GRK2, as demonstrated with a proximity ligation assay. Light elicited an intensity-dependent relaxation of PAs preconstricted with phenylephrine (PE), with a maximum response between 400 and 460 nm (blue light). Wavelength-specific photorelaxation was attenuated in PAs from Opn4 -/- mice and further reduced following shRNA-mediated knockdown of Opn3. Inhibition of GRK2 amplified the response and prevented physiological desensitization to repeated light exposure. Blue light also prevented PE-induced constriction in isolated PAs, decreased basal tone, ablated PE-induced single-cell contraction of PASMCs, and reversed PE-induced depolarization in PASMCs when GRK2 was inhibited. The photorelaxation response was modulated by soluble guanylyl cyclase but not by protein kinase G or nitric oxide. Most importantly, blue light induced significant vasorelaxation of PAs from rats with chronic pulmonary hypertension and effectively lowered pulmonary arterial pressure in isolated intact perfused rat lungs subjected to acute hypoxia. These findings show that functional Opn3 and Opn4 in PAs represent an endogenous "optogenetic system" that mediates photorelaxation in the pulmonary vasculature. Phototherapy in conjunction with GRK2 inhibition could therefore provide an alternative treatment strategy for pulmonary vasoconstrictive disorders.