Open AccessAnalysis of Hydroxyl Radical and Hydrogen Peroxide Generated in Helium-Based Atmospheric-Pressure Plasma Jet and in Different Solutions Treated by Plasma for Bioapplications
Author(s) -
Tsung Wen Chen,
Chih Tung Liu,
Chao Yu Chen,
Mu Chien Wu,
Po Chien Chien,
YiMing Cheng,
Jingwei Wu
Publication year - 2020
Publication title -
ecs journal of solid state science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.488
H-Index - 51
eISSN - 2162-8777
pISSN - 2162-8769
DOI - 10.1149/2162-8777/ab9c78
Subject(s) - hydrogen peroxide , terephthalic acid , atmospheric pressure plasma , hydroxyl radical , plasma , materials science , hydrogen , radical , analytical chemistry (journal) , atmospheric pressure , helium , nuclear chemistry , chemistry , chromatography , organic chemistry , polyester , physics , oceanography , quantum mechanics , geology , composite material
This study systematically analyzed reactive species generated with self-built helium-based low-temperature atmospheric-pressure plasma jet (He-APPJ); we measured the hydroxyl radical (·OH) and hydrogen peroxide (H 2 O 2 ) above surfaces of plasma–treating solutions and in plasma-treated medium (PTM) of different solutions, including deionized water, phosphate buffered saline, and Dulbecco’s modified Eagle medium. The effects of adding O 2 into He working gas on ·OH and H 2 O 2 generation were discussed. The MCF7 cell responses to plasma treatment were also observed. The ·OH emission (309 nm) in He-APPJ and above surfaces of plasma–treating solutions were analyzed through optical emission spectroscopy, and concentrations of ·OH and H 2 O 2 in different solutions were measured using terephthalic acid and Amplex Red, respectively. In our plasma system, it was observed that higher conductivity of solutions may cause stronger ·OH emission above solution. The ingredients of the solution may affect the concentrations of ·OH and H 2 O 2 . Addition of 0.1% O 2 generates strongest ·OH emission above surfaces of plasma–treating solution with our He-APPJ, and thus, the highest H 2 O 2 concentration in PTM. The MCF7 cell viability is influenced by the H 2 O 2 concentration in PTM. The results of this study can facilitate further study of plasma effects on PTM and cell culture.