Open AccessTowards an Electrochemical Immunosensor for the Detection of Antibodies against SARS-CoV-2 Spike Protein
Author(s) -
Viktorija Liustrovaitė,
Maryia Drobysh,
A. Ručinskienė,
Aušra Baradokė,
Ieva Plikusienė,
Almira Ramanavičienė,
Urtė Samukaitė-Bubnienė,
Roman Viter,
Chuan-Mu Chen,
Arūnas Ramanavičius
Publication year - 2022
Publication title -
journal of the electrochemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.258
H-Index - 271
eISSN - 1945-7111
pISSN - 0013-4651
DOI - 10.1149/1945-7111/ac5d91
Subject(s) - dielectric spectroscopy , detection limit , cyclic voltammetry , electrochemistry , antibody , coronavirus , recombinant dna , chemistry , chromatography , electrode , covid-19 , biochemistry , medicine , infectious disease (medical specialty) , immunology , disease , pathology , gene
Herein we report the electrochemical system for the detection of specific antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins in blood serum patient samples after coronavirus disease 2019 (COVID-19). For this purpose, the recombinant SARS-CoV-2 spike protein (SCoV2-rS) was covalently immobilised on the surface of the gold electrode pre-modified with mixed self-assembled monolayer (SAMmix) consisting of 11-mercaptoundecanoic acid and 6-mercapto-1-hexanol. The affinity interaction of SCoV2-rS with specific antibodies against this protein (anti-rS) was detected using two electrochemical methods: cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The anti-rS was detected with a detection limit of 2.53 nM and 1.99 nM using CV and EIS methods, respectively. The developed electrochemical immunosensor is suitable for the confirmation of COVID-19 infection or immune response in humans after vaccination.