Open AccessA Novel Electrochemical-Biosensor Microchip Based on MWCNTs/AuNPs for Detection of Glycated Hemoglobin (HbA1c) in Diabetes Patients
Author(s) -
Khanittha Ponsanti,
Nipaporn Ngernyuang,
Benchamaporn Tangnorawich,
Kesara NaBangchang,
Kanyarat Boonprasert,
Adis Tasanarong,
Teerachat Saeheng,
Akkaracha Hanwattanakula,
Chiravoot Pechyen
Publication year - 2022
Publication title -
journal of the electrochemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.258
H-Index - 271
eISSN - 1945-7111
pISSN - 0013-4651
DOI - 10.1149/1945-7111/ac5c0a
Subject(s) - colloidal gold , glycated hemoglobin , materials science , electrode , nanoparticle , hemoglobin , biosensor , carbon nanotube , electrochemistry , nanotechnology , biomedical engineering , diabetes mellitus , type 2 diabetes , chemistry , medicine , organic chemistry , endocrinology
This study reports an electrochemical sensor based on multiwalled carbon nanotubes/gold nanoparticles (MWCNTs/AuNPs) for detection of total hemoglobin (Hb) and glycated hemoglobin (HbA1c) in blood samples. Measurement of glycated hemoglobin percentage (%HbA1c) is crucial to the monitoring of glycemia in diabetes patients. The %HbA1c, which refers to the concentration ratio of HbA1c to total hemoglobin (Hb), has been widely used for accurate determination of stage-wise diabetes. The gold nanoparticles (AuNPs) used in the sensor were synthesized using a biological method employing passion fruit peel extracts. The resulting AuNPs are spherical with diameter approximately 18 nm, and these can be mixed with MWCNTs in various ratios to form MWCNTs/AuNPs composites. TEM imaging indicates that gold nanoparticles decorate the surface of the MWCNTs. Coating of a screen-printed carbon electrode (SPCE) with MWCNTs/AuNPs affords the novel MWCNTs/AuNPs/SPCE electrode, which exhibits a linear response over a 0.186–2.044 g dl −1 HbA1c concentration range, with the correlation value of 0.96. The electrode can reliably be utilized for detection of total Hb and HbA1c in blood samples, which highlights its promise as a reliable approach for sensitive point-of-care testing (POCT) of HbA1c in diabetes patients.