Open AccessTerminal Deoxynucleotidyl Transferase Extension-Dominated In Situ Signal Attenuation-Free Electrochemical Platform and Its Logic Gate Manipulation
Author(s) -
Qi Wang,
Tingting Hao,
Kai-Xin Hu,
Lingxia Qin,
Xinxin Ren,
Zhiyong Guo,
Sui Wang,
Yufang Hu
Publication year - 2022
Publication title -
journal of the electrochemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.258
H-Index - 271
eISSN - 1945-7111
pISSN - 0013-4651
DOI - 10.1149/1945-7111/ac4a54
Subject(s) - terminal deoxynucleotidyl transferase , signal (programming language) , ohmic contact , cyclic voltammetry , potentiostat , electrochemistry , materials science , chemistry , analytical chemistry (journal) , optoelectronics , computer science , biological system , electrode , biochemistry , tunel assay , apoptosis , chromatography , biology , programming language
Signal generation of traditional electrochemical biosensors suffers from the random diffusion of electroactive probes in a electrolyte solution, which is accompanied by poor reaction kinetics and low signal stability from complex biological systems. Herein, a novel circuit system with autonomous compensation solution ohmic drop (noted as “fast-scan cyclic voltammetry (FSCV)”) is developed to solve the above problems, and employed to achieve terminal deoxynucleotide transferase (TdT) and its small molecule inhibitor analysis. At first, a typical TdT-mediated catalytic polymerization in the conditions of original DNA, deoxythymine triphosphate (dTTP) and Hg 2+ is applied for the electrode assembly. The novel electrochemical method can provide some unattenuated signals due to in situ Hg redox reaction, thus improving reaction kinetics and signal stability. This approach is mainly dependent on TdT-mediated reaction, so it can be applied properly for TdT investigation, and a detection limit of 0.067 U ml −1 ( S / N = 3) is achieved successfully. More interesting, we also mimic the function of TdT-related signal communication in various logic gates such as YES, NOT, AND, N-IMPLY, and AND-AND-N-IMPLY cascade circuit. This study provides a new method for the detection of TdT biomarkers in many types of diseases and the construction of a signal attenuation-free logic gate.