High-Speed Atomic Force Microscopy | Zendy

Toshio Ando | Zendy; Takayuki Uchihashi | Zendy; Noriyuki Kodera | Zendy

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High-Speed Atomic Force Microscopy

Author(s) -

Toshio Ando,

Takayuki Uchihashi,

Noriyuki Kodera

Publication year - 2012

Publication title -

japanese journal of applied physics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.487

H-Index - 129

eISSN - 1347-4065

pISSN - 0021-4922

DOI - 10.1143/jjap.51.08ka02

Subject(s) - biomolecule , bacteriorhodopsin , atomic force microscopy , nanotechnology , microscopy , molecule , temporal resolution , protein filament , chemistry , materials science , physics , optics , biochemistry , organic chemistry , membrane

The technology of high-speed atomic force microscopy (HS-AFM) has reached maturity. HS-AFM enables us to directly visualize the structure and dynamics of biological molecules in physiological solutions at subsecond to sub-100 ms temporal resolution. By this microscopy, dynamically acting molecules such as myosin V walking on an actin filament and bacteriorhodopsin in response to light are successfully visualized. Highresolution molecular movies reveal the dynamic behavior of molecules in action in great detail. Inferences no longer have to be made from static snapshots of molecular structures and from the dynamic behavior of optical markers attached to biomolecules. In this review, we first describe theoretical considerations for the highest possible imaging rate, then summarize techniques involved in HS-AFM and highlight recent imaging studies. Finally, we briefly discuss future challenges to explore. © 2012 The Japan Society of Applied Physics

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