Open AccessNoninvasive in vivo study of NADH fluorescence and its real-time intrinsic dynamical changes: Experiments and seven-layered skin model Monte Carlo simulations
Author(s) -
Huayi Hou,
Gongzhi Du,
Yuancheng Wang,
Chunping Su,
Lianbo Guo,
XiangBai Chen
Publication year - 2022
Publication title -
journal of innovative optical health sciences/journal of innovation in optical health science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 24
eISSN - 1793-5458
pISSN - 1793-7205
DOI - 10.1142/s1793545822300063
Subject(s) - fluorescence , biophysics , nicotinamide adenine dinucleotide , chemistry , hyperaemia , in vivo , monte carlo method , human skin , biological system , nad+ kinase , biochemistry , optics , physics , blood flow , biology , enzyme , medicine , microbiology and biotechnology , statistics , mathematics , genetics
Reduced nicotinamide adenine dinucleotide (NADH) plays a crucial role in many biochemical reactions in human metabolism. In this work, a flow-mediated skin fluorescence (FMSF)–post-occlusion reactive hyperaemia (PORH) system was developed for noninvasive and in vivo measurement of NADH fluorescence and its real-time dynamical changes in human skin tissue. The real-time dynamical changes of NADH fluorescence were analyzed with the changes of skin blood flow measured by laser speckle contrast imaging (LSCI) experiments simultaneously with FMSF-PORH measurements, which suggests that the dynamical changes of NADH fluorescence would be mainly correlated with the intrinsic changes of NADH level in the skin tissue. In addition, Monte Carlo simulations were applied to understand the impact of optical property changes on the dynamical changes of NADH fluorescence during the PORH process, which further supports that the dynamical changes of NADH fluorescence measured in our system would be intrinsic changes of NADH level in the skin tissue.