Open AccessObservations of intracellular second-harmonic generation imaging in black phosphorus nanosheets
Author(s) -
Xiao Peng,
Yiping Song,
Peng Zheng,
Kaixuan Nie,
Hao Líu,
Ying Zhou,
Feifan Zhou,
Yuan Yua,
Jun Song,
Junle Qu
Publication year - 2020
Publication title -
journal of innovative optical health sciences/journal of innovation in optical health science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 24
eISSN - 1793-5458
pISSN - 1793-7205
DOI - 10.1142/s1793545820410060
Subject(s) - photothermal therapy , nanoprobe , intracellular , materials science , in vivo , second harmonic generation , hela , black phosphorus , excitation , biophysics , second harmonic imaging microscopy , laser , nanotechnology , optoelectronics , in vitro , chemistry , nanoparticle , optics , biology , biochemistry , physics , microbiology and biotechnology , electrical engineering , engineering
Functionalized black phosphorus (BP) nanosheets have been considered as promising nanoagents in cancer therapy due to their excellent photothermal conversion efficiency. However, it is still difficult to visually monitor the dynamic localization of BP nanoagents in cancer cells. In this paper, we systematically studied the second-harmonic generation (SHG) signals originating from exfoliated BP nanosheets. Interestingly, under the excitation of a high frequency pulsed laser at 950 nm, the SHG signals of BP nanosheets in vitro are almost undetectable because of their poor stability. However, the intracellular SHG signals from BP nanosheets could be measured by in vivo optical [Formula: see text]imaging due to the efficient enrichment of living HeLa cells. Moreover, the SHG signal intensity from BP nanosheets increases with the prolonged incubation time. It can be expected that the BP nanosheets could be a promising intracellular SHG nanoprobe employed for visually in vivo biomedical imaging in practical cancer photothermal therapy (PIT).