Open AccessIn vivo mice brain microcirculation monitoring based on contrast-enhanced SD-OCT
Author(s) -
Shaozhuang Yang,
Liwei Liu,
Ye Chang,
Ningning Zhang,
Kun Liu,
Hong Liang,
Bingling Chen,
Yue Zhao,
Rui Hu,
Junle Qu
Publication year - 2019
Publication title -
journal of innovative optical health sciences/journal of innovation in optical health science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 24
eISSN - 1793-5458
pISSN - 1793-7205
DOI - 10.1142/s1793545819500019
Subject(s) - microcirculation , in vivo , optical coherence tomography , polyethylene glycol , biomedical engineering , contrast (vision) , peg ratio , materials science , chemistry , biophysics , medicine , optics , radiology , biology , physics , microbiology and biotechnology , organic chemistry , finance , economics
In this paper, we proposed a contrast-enhanced homemade spectral domain optical coherence tomography (SD-OCT) method for monitoring of brain microcirculation. We used the polyethylene glycol (PEG)-ylated gold nanorods (GNRs) as a contrast-enhanced agent, obtained clearly 2D and 3D OCT images of blood vessels and dynamic changes of probes in mouse blood vessels. Owing to high scattering of the PEG-GNRs, more tiny blood vessels can be imaged and the OCT signal can be enhanced by 5.87 dB after injection of PEG-GNRs for 20[Formula: see text]min, the enhancement then declined gradually for 60[Formula: see text]min. Our results demonstrate an effective technique for the enhanced imaging of blood vessels in vivo, especially for studies of the brain microcirculation, which could be serviced for disease mechanism research and therapeutic drug monitoring.